FluOMICS, in partnership with the FluDyNeMo consortium and the Innate Immune Responses and Vaccines against Tumor-associated Herpesviruses P01 grant, hosted a day-long symposium at the Sanford Consortium for Regenerative Medicine on January 23, 2017 to highlight recent advances and breakthroughs in the field of systems research of infectious disease. Speakers included Drs. Sara Cherry, Paul Thomas, Michael Gale, Reed Shabman, Jeff Cox, Betsy Herold, Gustavo Palacios, Jacques Banchereau, Robert Davey, Pei-Yong Shi, Charlie Rice, as well as two talks given by postdoctoral fellows in the Program, Judd Hultquist (UCSF) and Laura-Isobel McCall (UCSD). The talks were followed by a poster session and reception. Dr. Kelsey Haas (UCSF) received a travel grant to help cover travel costs to the Symposium.
This conference brought together nationally and internationally renowned scientists with those from across the San Diego Torrey Pines Mesa who are focused on the study of small molecule metabolites and metabolic biochemistry. The meeting included investigators with interest in technical mass spectrometry, analytical chemistry and the biological applications of these emerging tools. Speakers consisted of Mohit Jain, UCSD (organizer); Gary Siuzdak, Scripps; Christian Metallo, UCSD; Sophia Lunt, Michigan State University; Kumar Sharma, UCSD; Pieter Dorrestein, UCSD; Rany Salem, UCSD and Daniel Nomura, UC Berkeley. Hosted by SDCSB and FluOMICS.
Pooled CRISPR based screens have become an important tool for functional genomics. Multiple laboratories in La Jolla are initiating screens and developing standard operating procedures for screen design, execution and analysis. The goal of the workshop was to provide attendees with the tools and knowledge to design, execute and analyze screens leading to reliable, reproducible and sensitive results. Speakers included Olivier Harismendy (organizer), Ana Bojorquez, John Paul Shen, Philipp Spahn, Eric Van Nostrand and Kristen Jepsen of UCSD. Hosted by SDCSB in partnership with FluOMICS.
In this workshop, organized by SDCSB and FluOMICS, the Cytoscape development team used a completely new format to provide a hands-on tutorial in an intimate classroom setting. Attendees at the yearly spring workshop had previously requested a non-lecture format where individuals can pose problems that will be directly addressed by Cytoscape tutors and developers. The fall workshop was held as a clinic, where Cytoscape users signed up for 15 minute slots where he/she presented a problem (preferentially with actual data), and Cytoscape tutors, developers, and users were able to address it directly on the spot. Hosted by Barry Demchak, UCSD.
Cytoscape is the most popular software for visualizing molecular networks and biological pathways and for integrating these networks with annotations, gene expression profiles and other state data. Although Cytoscape was originally designed for biological research, now it is a general platform for complex network analysis and visualization. In this workshop, the Cytoscape development team from Trey Ideker’s lab presented a series of lectures and hands-on demonstrations covering a range of issues for both beginning and advanced users. Speakers included Barry Demchak (organizer), Keiichiro Ono and Dexter Pratt of UCSD.
Organized by Dr. Kamala Janiyani of UCSD, this workshop brought together intellectual property professionals, entrepreneurs, researchers and students to discuss the recent developments in the area of intellectual property and licensing. Invited speakers included Greg Reilly, California Western School of Law; James Herkenhoff, Knobbe Martens; John Cabeca, USPTO; Matthew Brown, BD Biosciences; Tracie Carroll, SBPMDI; Victoria Cajipe, UCSD; Scott M Iyama, Orrick, Herrington and Sutcliffe LLP and Mridula Pottathil Sherin, UCSD.
The goal of this project is to develop a novel tool for the study of mRNA degradation rates in the presence of an active IAV infection. We will then apply this new technique to obtain the first comprehensive view of transcriptome stability dynamics during infection. Through bioinformatic analysis of this new and existing data from the U19 consortium we will be able to determine cis-acting mRNA sequences that are targeted and the overall role degradation plays in modulation of the host environment. Specific Aims are (1) to develop novel tools to study intrinsic host mRNA stability throughout the IAV life cycle in vitro, and (2) to determine mRNA stability at a global level in IAV-infected cells.
The NIAID-funded Systems Biology Centers, FluOMICS and FluDyNeMo Programs, in collaboration with San Diego Center for Systems Biology (SDCSB) hosted the 2nd Annual "Host-Pathogen Interactions: Circuits to Systems" Symposium. The symposium brought together scientist from diverse disciplines with presentations covering application of systems biology to study viral and bacterial infections, modeling of biological networks, and development of computational resources. Lauren Aguado, Kelsey Haas, Marie-Theres Pohl, Michael Schotseart, and Ana Veiga were selected as recipients of travel awards to attend the symposium.
A new version of our Gene Annotation and Analysis tool “Metascape” has been released, now implementing best practices for multi-list meta-analyses.
The 2nd Annual “Host-Pathogen Interactions: Circuits to Systems" Symposium will be hosted by the NIAID-funded FluOMICS and FluDyNeMo Programs in collaboration with SDCSB.
Elucidating the molecular mechanisms that govern host-pathogen interactions is a subject of intense research in the field of infectious disease. National and local thought leaders will discuss current gaps in our understanding of how pathogens manage to evade host immune surveillance and establish acute and chronic infection states. Cutting edge systems biology approaches will be highlighted to describe recent advances in molecular and systems analyses of microbial-host interactions, disease pathogenesis, and immune responses. The Symposium is a day-long event on January 4, 2016 at the Sanford Consortium for Regenerative Medicine in La Jolla, California. The speaker lineup includes Drs. Eva Harris, Daniel DiMaio, Eva Friebertshäuser, Bert Semler, Victor Nizet, Yingyao Zhou, Rafick Sekaly, Sean Whelan, Ivan Marazzi and Stuart Sealfon. Participation is FREE, but registration is required for all attendees. Deadline to submit abstracts and travel grant applications is December 7th, 2015. Link
We have just launched Metascape, a new gene annotation and analysis tool. The aim of Metascape is to develop a set of reliable, productive and intuitive tools that help biomedical research community to analysis gene/protein lists and make better data-driven decisions. Although a plethora of databases and tools exist for gene annotation and gene list enrichment analysis, it remains challenging for researchers, especially those who are not trained to programming, to gather some very basic annotation data for their list of genes.
The Metascape web site and tools enable users to apply popular bioinformatics analyses to one or multiple gene/protein lists in order to make effective data-driven gene prioritization decisions. Just with a few mouse clicks, we can start from lists of gene/protein identifiers, extract rich annotations and identify statistically enriched pathways. http://www.metascape.org/
We would like to announce that a new pilot project has been awarded through the FluOMICS program:
The mass spectrometric analysis of complex protein mixtures is often hindered by a high degree of complexity. Abundant proteins dominate the mass spectrometer’s duty cycle producing spectra that are highly redundant and obscure the detection of less abundant proteins. This proposal aims to develop a novel technology to simplify complex protein mixtures by isolating partially digested N-terminal protein fragments. The successful implementation of such a method will increase the coverage of the proteome that may be captured and reduce the instrument time required to obtain a comprehensive snapshot of the proteome. Eliminating fractionation methods for proteome analysis will also allow for label-free quantification methods to be applied to characterize protein dynamics with high sensitivity and in biologically relevant systems with ease.
Workshop: Quantitative Proteomics Approaches to Study Host-Pathogen Relationships.
In partnership with SDCSB, a best-practices Workshops in Proteomics will be organized by Jeff Johnson of the Krogan lab on September 10th at the SCRM. This workshop will focus on the best practices in the field for applying quantitative proteomics approaches to study host-pathogen relationships. Speakers will describe the latest and most effective methods for probing protein-protein interactions, post-translational modifications, as well as advanced approaches to add quantitative value to proteomics studies. Special attention will be placed on experimental design and bioinformatics strategies for each approach that maximize the amount of biological information that can be extracted from highly complex proteomics data. http://sdcsb.ucsd.edu/proteomics-2016/
In July, FluOMICS successfully co-hosted together with SDCSB and Dr. Lev Tsimring of UCSD the La Jolla Labs Quantitative Biology summer course (http://www.lajollalabs.org). The course included chalkboard lectures on mathematical modeling, an integrated computer lab for hands-on exploration and research presentations from leaders in the field, including host-pathogen interactions. Fluomics will again co-host this course in the summer of 2016. Further details of the course program will be available in early 2016.
Announcement of upcoming SDCSB/FluOMICS co-hosted workshops:
Metabolomics Workshop on March 27, 2015, organized by Mohit Jain, an Asst Prof at the UCSD School of Medicine
For registration and detailed information, visit http://sdcsb.ucsd.edu/
We would like to announce that two pilot projects have been awarded through the FluOMICS program:
Sumit Chanda, Sanford Burnham Instititue. La Jolla, CA
The goal of this project is to develop and execute a high-throughput screen to assess alterations in host protein subcellular localization after influenza infection. To address the hypothesis that viruses retarget host protein distribution to evade immune detection, we propose to establish and optimize a high-throughput screening platform to interrogate subcellular (trans)localization events. The screen to be performed will evaluate the impact of influenza infection on the localization of ~500 host proteins that encode a variety of genes related to pattern recognition, interferon signaling and interferon-dependent target genes. To facilitate the highthroughput approach, epitope-tagged cDNAs will be transduced into cells, and after viral challenge, cells will be fixed, stained, and imaged utilizing high-content microscopy. Refined automated imaging analysis algorithms will enable us to computationally identify differential localization events. Validation and kinetic analysis of trafficking events targeted by influenza. To assess the biological relevance of candidate genes identified in SA1, we will assess the translocation events of endogenous proteins (when antibodies are available), as well as perform kinetic analyses of translocation with RFP-tagged proteins (3-5 genes).